Discussion Overview
The discussion revolves around the feasibility of using reverse osmosis (RO) water as a substitute for MilliQ water in the preparation of buffers for molecular biology applications. The context includes considerations of water purity, laboratory protocols, and the implications of using different water types in experimental settings.
Discussion Character
- Debate/contested
- Technical explanation
- Conceptual clarification
Main Points Raised
- One participant questions whether RO water can replace MilliQ water, noting the difference in conductivity between the two.
- Another participant suggests that using RO water may be acceptable as long as it aligns with the reporting protocols, emphasizing the importance of transparency in documentation.
- A participant clarifies that their lab has a MilliQ system but is temporarily unable to use it due to maintenance issues, prompting the consideration of RO water as an alternative.
- There is a suggestion that if protocols only "suggest" the use of MilliQ water, then RO water might be an acceptable substitute, provided it is reported accordingly.
- One participant advises maintaining spare parts for critical lab equipment to avoid such situations in the future.
Areas of Agreement / Disagreement
Participants express differing views on the necessity of using MilliQ water versus RO water. While some argue that RO water may be acceptable under certain conditions, others emphasize the importance of adhering to established protocols. The discussion remains unresolved regarding the strict requirements for water purity in this context.
Contextual Notes
The discussion highlights the lack of clarity in laboratory protocols regarding the necessity of using MilliQ water, as well as the implications of using RO water in terms of reproducibility and reliability of results.