Why Does Fluorescence Decrease During the Lag Phase in 3T3 Cell Growth Curves?

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SUMMARY

The fluorescence values in 3T3 cell growth curves decrease during the lag phase, which is an unexpected observation. The discussion suggests that this decrease may not be attributed to cell death outpacing replication, as it occurs between days 1 and 3. Participants recommend conducting statistical analysis to determine if the decrease is statistically significant and to assess whether it is simply normal variance or experimental noise. Raw data provision is also encouraged for further investigation.

PREREQUISITES
  • Understanding of 3T3 cell culture techniques
  • Knowledge of fluorescence measurement methods
  • Familiarity with statistical analysis in biological experiments
  • Experience with data interpretation in cell biology
NEXT STEPS
  • Conduct statistical analysis on fluorescence data to assess significance
  • Research normal variance in biological measurements
  • Explore methods for improving fluorescence measurement accuracy
  • Investigate the lag phase characteristics in cell growth dynamics
USEFUL FOR

Cell biologists, researchers studying cell growth dynamics, and laboratory technicians involved in fluorescence measurements will benefit from this discussion.

Redmac
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Hi All,

Having plotted a cell growth curve (fluorescence vs days), I have found that during the lag phase, the fluorescence values decrease. I haven't come across this previously during lectures and can't seem to find any examples online to help explain it. My only thought was that cell death occurred at a higher rate than replication whilst the cells were attaching to the culture flask but the decrease occurs between 1 and 3 days so this doesn't seem like a possible explanation. As a side note, 3T3 cells were being used during the experiment.

I'd appreciate any advice on this or resources that may be useful.

Many Thanks
 
Have you run statistical analysis on the fluorescence values? Is it statistically significant decrease? Can you provide the raw data? It could just be normal variance or noise that you're interpreting as a decrease.
 

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