Detection limits for Raman spectroscopy

In summary, the conversation discusses the feasibility of using Raman spectroscopy to detect chemicals in human tissue samples. The main concerns raised are the penetration depth and the efficiency of interpreting the data. However, it is possible to apply Raman spectroscopy to tissue samples in forensic science and there have been studies on the Raman spectra of chemicals in human tissue. The main limitation would be the minimum detection limit for low concentrations of chemicals.
  • #1
penofguino
5
0
This may be a completely terrible question, but does someone have an idea on the detection capabilities for raman spectroscopy of a bulk sample like human tissue (hair, blood, skin, anything)?

I thought it might be fun to see if it were possible to use raman to identify exposures to chemicals like bisphenol-A etc by measuring a tissue sample, but I did not know if this was too far beyond current capabilities.
 
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  • #2
The bulk limiting factor is the ability to penetrate the sample, and in forensic applications you would be using near and mid-IR spectroscopy to achieve this. The other main question is efficiency, vibrational spectroscopy methods like this don't require labeling or staining of the biomaterial but instead specialists who are able to apply the mathematical tools and time necessary to interpret the large amount of data. Would it be possible though, definitely. Methods for applying Raman spectroscopy to tissue samples in forensic science have been around for decades and the Raman spectra of bisphenol-A is wholly studied.
 
  • #3
Thanks for your reply!

The penetration depth is obviously an issue, but that would correspond with an increase in any minuimum detection limit. Is there any good examples of studies looking at certain concentrations of a chemical in human tissue? For environmental exposures in general, you would have very low concentrations of ppm or ppb levels.
 

FAQ: Detection limits for Raman spectroscopy

1) What is the significance of detection limits in Raman spectroscopy?

Detection limits in Raman spectroscopy refer to the smallest amount of a substance that can be detected and accurately measured by the instrument. This is an important factor to consider when analyzing samples, as it determines the sensitivity and reliability of the results.

2) How are detection limits determined in Raman spectroscopy?

Detection limits are typically determined by analyzing a known concentration of a substance and measuring the signal-to-noise ratio. The limit of detection is usually defined as the concentration at which the signal is three times higher than the background noise.

3) What factors can affect the detection limits in Raman spectroscopy?

The detection limits in Raman spectroscopy can be affected by various factors such as sample concentration, instrument sensitivity, laser power, and sample matrix. The type of sample and the presence of interfering substances can also impact the detection limits.

4) Can detection limits be improved in Raman spectroscopy?

Yes, the detection limits in Raman spectroscopy can be improved by optimizing instrument parameters, such as increasing the laser power or using a more sensitive detector. Pre-concentration techniques and sample preparation methods can also help to improve the detection limits.

5) How important are detection limits in Raman spectroscopy for real-world applications?

Detection limits are crucial in real-world applications of Raman spectroscopy, especially in fields such as forensics, pharmaceuticals, and environmental analysis. Low detection limits allow for the detection of trace amounts of substances, which is important for accurate and reliable analysis of samples.

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