Does muscle have the least protein content?

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Discussion Overview

The discussion revolves around the protein content and other properties of various biological tissues, specifically in the context of an experiment involving pulsed nuclear magnetic resonance spectroscopy. Participants explore how properties like spin-spin relaxation time (T2) vary among muscle, liver, fat, heart, cartilage, and bone marrow.

Discussion Character

  • Exploratory, Technical explanation, Debate/contested

Main Points Raised

  • One participant questions whether muscle has the least protein content compared to other tissues like liver and bone marrow, and whether the size of molecules varies in the same order as the T2 measurements.
  • Another participant emphasizes the importance of details regarding the measurements and sample preparation in understanding the results.
  • A participant explains that the experiment measures the spin-spin relaxation time T2, which increases in a specific order for the biological samples, prompting questions about the underlying biological reasons for this trend.
  • It is suggested that fat has a short T2 due to efficient energy exchange, while water, being less efficient, contributes to a longer T2. This leads to a consideration of the water-to-fat ratio in different tissues.
  • One participant notes an observation that fatty tissue has a shorter T2 than bone marrow, which is expected to have more water.

Areas of Agreement / Disagreement

Participants express differing views on the relationship between protein content and T2 values, and there is no consensus on the specific properties that change in the order of the tissues mentioned.

Contextual Notes

The discussion lacks detailed definitions of the properties being measured and does not resolve the implications of the varying T2 values among the tissues.

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I am in the middle of a physics experiment that has recently crossed over into biology...the details are not important, but what i found was that a certain property changed in this order as the following substances were scanned:

1. muscle
2. liver
3. fat
4. heart
5. cartilage
6. bone marrow

is there any biological or physical property (density, protein content, etc??) that would change in that order? e.g. does muscle have the least protein content and bone marrow the most? or vice versa? or is it maybe the size of the molecules that vary in that order?
 
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the details are important what are you measuring en what is your sample preparation¿
 
Its an experiment on pulsed nuclear magnetic resonance spectroscopy and the factor that increases (roughly linearly for some reason) with muscle, liver...marrow is the spin spin relaxation time T2

so I placed small samples of each type of tissue, scanned them, found an average relaxation time and standard error for each tissue, and plotted them...

now my question is, why does T2 increase in that fashion for the biological samples...what is it about liver for instance that causes a higher T2 than muscle
 
Fat has a very efficient energy exchange and therefore it has a relatively short T2.
Water is less efficient than fat in the exchange of energy, and therefore it has a long T2 time
So, I would look at ratio of water to fat in these various tissues. I note that your "fatty tissue" has shorter T2 than your bone marrow which should have relatively more water. Just a thought--good luck.
 

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