ELISA Tests and Contagion: What You Need to Know

  • Thread starter Thread starter Echo
  • Start date Start date
  • Tags Tags
    Test
Click For Summary

Discussion Overview

The discussion revolves around the safety and contagion risk associated with ELISA tests, particularly in a school setting. Participants explore concerns related to personal safety during the testing process and the nature of the materials used in the test kits.

Discussion Character

  • Exploratory, Technical explanation, Debate/contested, Conceptual clarification

Main Points Raised

  • One participant expresses concern about feeling ill after performing an ELISA test without gloves, questioning the potential for contagion from the test.
  • Another participant asserts that ELISA tests are safe and should not be contagious, asking for clarification on the sample used in the test.
  • A participant explains that the antigen in the test kit consists of purified EBV proteins, which should not pose a contagion risk, contrasting this with the risks associated with handling infected body fluids.
  • One participant emphasizes the safety of ELISA tests compared to older methods like radioimmunoassays, suggesting that illness is more likely due to common colds or flu spread among students.

Areas of Agreement / Disagreement

Participants generally agree that ELISA tests are safe and not contagious when proper materials are used. However, there is some uncertainty regarding the specific circumstances of the test performed and the potential for illness from other sources.

Contextual Notes

Participants mention the importance of using gloves and proper techniques during testing, indicating a recognition of safety protocols, but do not resolve the implications of personal health concerns raised.

Who May Find This Useful

Individuals interested in laboratory safety, ELISA testing procedures, and the implications of handling biological samples may find this discussion relevant.

Echo
Messages
5
Reaction score
0
I had to do an ELISA test in school, for EBV. I forgot to put gloves on :S

So now, about 5 days later, I'm starting to feel kinda ill, like flu or strep.. Throat hurts a bit, I'm pretty tired etc.
Could the ELISA test in any way be contagious? I don't quite understand if it could :(
 
Biology news on Phys.org
No, otherwise it would be very irresponsible to use it in a school setting. What was the sample that was used for the test?

Remember to always wear gloves and wash your hands after doing experiments.
 
I think it was a test kit the teacher got, which included antigen, antibody, serum and substrate.. Yeah I didn't think it would be very responsible either :P But I had to know.
 
Echo said:
I think it was a test kit the teacher got, which included antigen, antibody, serum and substrate.. Yeah I didn't think it would be very responsible either :P But I had to know.

Ok, so the antigen of the kit is the 'sample'. This will be purified pieces of EBV proteins, which should not be contagious. There is nothing for you to worry about. The case would have been different if you had been working with actual infected body fluids, which you haven't.

Always work very carefully. I've heard of a case where a student got blood in her mouth from a patient, because she used a mouth pipet instead of an automatic one. You really do not want to make these kinds of mistakes.
 
Okay, thanks a lot :)
 
The whole point of using an ELISA is they are MUCH safer than radioimmunoassays that they have replaced. Nothing there but sandwiches of antibodies and some color tags on them.

More likely, you're just catching whatever bug your fellow students are spreading around the old-fashioned way of coughing and sneezing. :wink:
 

Similar threads

  • · Replies 16 ·
Replies
16
Views
2K
  • · Replies 7 ·
Replies
7
Views
2K
  • · Replies 9 ·
Replies
9
Views
2K
  • · Replies 287 ·
10
Replies
287
Views
28K
  • · Replies 6 ·
Replies
6
Views
4K
  • · Replies 9 ·
Replies
9
Views
33K
  • · Replies 9 ·
Replies
9
Views
2K
Replies
4
Views
3K
  • · Replies 30 ·
2
Replies
30
Views
3K
  • · Replies 22 ·
Replies
22
Views
4K