To estimate the number of Cosmic Microwave Background (CMB) photons in 0.25 liters, one can start with the energy density formula for blackbody radiation, E = 4σ/C * T^4. The discussion highlights that there are approximately 411 CMB photons per cubic centimeter at present. To derive the number of photons, one can use the relationship E = hv, where v represents the frequency, and integrate over the photon distribution using Planck's Law. For those without access to specific textbooks, the photon gas Wikipedia page provides relevant formulas for calculating photon density based on temperature. Ultimately, using these principles will yield the desired estimate of CMB photons in the specified volume.