Experiment Exposing Tissue to Pulsed Electromagnetic Field

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Discussion Overview

The discussion revolves around the exposure of human tissue samples to pulsed electromagnetic fields (EMF) for experimental purposes in tissue engineering. Participants are exploring the parameters of the electromagnetic field, including pulse frequency, pulse width, and the appropriate range of EMF to use in their experiments.

Discussion Character

  • Exploratory
  • Technical explanation
  • Debate/contested
  • Experimental/applied

Main Points Raised

  • One participant describes their experimental setup involving a PIC16F886 generating pulses of 150 microseconds at a frequency of 80 Hz, seeking assistance in determining the EMF range for their work.
  • Another participant asks for clarification on the meaning of "active and deactivate pulse" and requests elaboration on the pulse width and cycle completion in the context of their experiment.
  • Questions are raised regarding the size and volume of tissue to be exposed to the AC magnetic field, as well as the instruments needed to drive the AC signal into the coil.
  • Concerns are expressed about the compatibility of the various frequency and pulse width numbers mentioned, prompting a request for references to the literature being consulted.
  • Participants inquire about the oversight of the project, including whether a Primary Investigator is involved and what medical experimental standards are being followed, as well as the nature of the subjects used in the experiments.

Areas of Agreement / Disagreement

The discussion contains multiple competing views and remains unresolved, particularly regarding the specifics of the experimental setup and the parameters for the pulsed EMF exposure.

Contextual Notes

Participants express uncertainty about the compatibility of different frequency and pulse width values, and there are missing details regarding the amplification of signals needed to drive the magnetic coil.

haslinda
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Hai guys,

My background is from tissue engineering more towards to biology. I am doing exposure of electromagnetic field to a human sample.

I have been assigned to use the magnetic device with the information as followed:
The PIC16F886 generates 150 microseconds (µs) of pulse frequency of 80 Hz. 80 Hz means there are 80 cycles of pulses in a T duration which equals to 12.5 milliseconds (ms), 6.25 ms each for active pulse and deactivate pulse. In a 6.25 ms duration, the 150 µs pulse width will took about 42 of pulsing cycles to complete. For second duration, the pulses will take about 6667 cycles to complete.

I really hope somebody can assist me , coz i have to decide the range of EMF that i should use in my work.
 
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haslinda said:
Hai guys,

My background is from tissue engineering more towards to biology. I am doing exposure of electromagnetic field to a human sample.

I have been assigned to use the magnetic device with the information as followed:
The PIC16F886 generates 150 microseconds (µs) of pulse frequency of 80 Hz. 80 Hz means there are 80 cycles of pulses in a T duration which equals to 12.5 milliseconds (ms), 6.25 ms each for active pulse and deactivate pulse. In a 6.25 ms duration, the 150 µs pulse width will took about 42 of pulsing cycles to complete. For second duration, the pulses will take about 6667 cycles to complete.

I really hope somebody can assist me , coz i have to decide the range of EMF that i should use in my work.
Welcome to PF. :smile:

Is this question for a schoolwork assignment? And what are you asking about -- Are you asking about how to calculate the value of the pulsed EM field? What is your transducer (magnetic coil, TENS electrodes, other)?
 
Hai, this is my experimental work. Actually, I want to use ranging from 0.2 mT – 5 mT between the frequency of 15-75 Hz on the proliferation. It was pulsed EMF. Can you help me to elaborate bout the description of the device. What is the meaning of active and deactive pulse. Then what is the meaning of "In a 6.25 ms duration, the 150 µs pulse width will took about 42 of pulsing cycles to complete".
 
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What size and volume of tissue do you want to expose this AC magnetic field to? What instrument(s) do you have to drive the AC signal into the coil with? You mention using a PIC16F886 to generate the waveforms, but those will be low-power logic signals, which need to be amplified to drive the coil that generates the magnetic field.

You mention lots of different numbers for frequency and pulse widths (not all of them compatible). Can you post a link to the reading you've been doing so far that has these numbers in it?

Finally, are you working for a Primary Investigator (PI) on this project? What medical experimental standards are you doing this testing to? Will you be using human volunteer subjects, or will you be using animals for the experiments?
 
berkeman said:
What size and volume of tissue do you want to expose this AC magnetic field to? What instrument(s) do you have to drive the AC signal into the coil with? You mention using a PIC16F886 to generate the waveforms, but those will be low-power logic signals, which need to be amplified to drive the coil that generates the magnetic field.

You mention lots of different numbers for frequency and pulse widths (not all of them compatible). Can you post a link to the reading you've been doing so far that has these numbers in it?

Finally, are you working for a Primary Investigator (PI) on this project? What medical experimental standards are you doing this testing to? Will you be using human volunteer subjects, or will you be using animals for the experiments?
Can i have your email sir
 
haslinda said:
Can i have your email sir
Nope. You can PM me if want to ask something private, but it's best to keep the discussion in the open forums. To send a PM, click on my Avatar and "Start a conversation"...
 
berkeman said:
Nope. You can PM me if want to ask something private, but it's best to keep the discussion in the open forums. To send a PM, click on my Avatar and "Start a conversation"...
ok i did reply to you
 

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