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Experimental Molecular Weight higher than Literature Value

1. Homework Statement
I just purified alkaline phosphatase, and my calculated molecular weight is 100000 g/mol, while literature value is 89000 g/mol. According to how others in my lab did calculations, my calculations are performed correctly, so I am left to explain why my purification is higher than expected.

2. Homework Equations
N/A

3. The Attempt at a Solution
I was thinking that since alkaline phosphatase can be inhibited by EDTA, my "purified" enzyme contains some EDTA, making the molecular weight higher.
 

Answers and Replies

chemisttree
Science Advisor
Homework Helper
Gold Member
3,080
61
Did you purify it by gel electrophoresis? What was the supporting electrolyte? Did you completely dialyze your product?
 
epenguin
Homework Helper
Gold Member
3,569
665
You do not give any indication of what method used for getting the MW so what can anyone say?
 

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