Fluorescent Microscopy: Can Antibodies Be Visualized?

  • Thread starter Thread starter TytoAlba95
  • Start date Start date
  • Tags Tags
    Microscopy
Join the discussion
Ask a follow-up here, or get your own question answered by working scientists, mathematicians and engineers — people, not an autocomplete.
Real named experts · corrections over time · the nuance an AI answer skips
2 replies · 2K views
TytoAlba95
Messages
132
Reaction score
19
TL;DR
Can a fluorescent tagged antibody raised against a protein be visualized under fluorescent microscope?
I'm aware that GFP tagged protein can be visualized under fluorescent microscopy. I don't know if it is possible with fluorescent tagged antibodies also. I did some research but couldn't find any information about this.

The answer provided is no.

(pg 25, sum no.142)
 
Biology news on Phys.org
Yes it can.
Fluorescent labeling with antibodies is a commonly used technique in many biology research labs.
Pictures.
There are a huge number of different techniques that can be used.

Similar technical principles will also apply to different labeling methods, such as labeling with biotin, enzymes or gold particles.
 
  • Like
Likes   Reactions: TytoAlba95
Thank you so much.