Fluorescent Microscopy: Can Antibodies Be Visualized?

[TytoAlba95]

TL;DR

Can a fluorescent tagged antibody raised against a protein be visualized under fluorescent microscope?

I'm aware that GFP tagged protein can be visualized under fluorescent microscopy. I don't know if it is possible with fluorescent tagged antibodies also. I did some research but couldn't find any information about this.

The answer provided is no.

(pg 25, sum no.142)

 

[BillTre]

Yes it can.
Fluorescent labeling with antibodies is a commonly used technique in many biology research labs.
Pictures.
There are a huge number of different techniques that can be used.

Similar technical principles will also apply to different labeling methods, such as labeling with biotin, enzymes or gold particles.

 

[TytoAlba95]

Thank you so much.