For those of you with the Alberts text

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SUMMARY

The discussion centers on the activation of DNA replication in the G1 phase of the cell cycle, specifically the role of the pre-replication complex (pre-RC) and the kinases involved. The key kinase mentioned is Cdc7p-Dbf4p, which collaborates with S-Cdk to phosphorylate the origin recognition complex (ORC) and initiate DNA replication. The ORC is crucial for the assembly of the pre-RC, which includes proteins such as Cdc6p, Cdt1p, and the minichromosome maintenance proteins (MCM). This process is essential for understanding the regulation of DNA replication in eukaryotic cells.

PREREQUISITES
  • Understanding of the cell cycle phases, particularly G1 phase.
  • Familiarity with protein kinases and their roles in cellular processes.
  • Knowledge of the pre-replication complex (pre-RC) and its components.
  • Basic concepts of DNA replication mechanisms in eukaryotes.
NEXT STEPS
  • Research the role of Cdc7p-Dbf4p in DNA replication initiation.
  • Explore the structure and function of the origin recognition complex (ORC).
  • Study the mechanisms of phosphorylation in cell cycle regulation.
  • Investigate the interactions between S-Cdk and other kinases during DNA replication.
USEFUL FOR

Cell biologists, molecular biologists, and researchers studying DNA replication mechanisms and cell cycle regulation will benefit from this discussion.

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For those of you with the Alberts text...

Page 999, top paragraph:

"Once the pre-RC has been assembled in G1, the replication origin is ready to fire . The activation of S-Cdk in late G1 pulls the trigger and initiates DNA replcation. The initiation of replication also requires the activity of a second protein kinase, which collaborates with S-Cdk to cause the phosphorylation of ORC."

My question: Is there a name for this mystery kinase? I can't seem to find anything on it.

Any help would be greatly appreciated. Thanks guys.
 
Biology news on Phys.org
In G1 phase (under low CDK activity), a protein complex called the “pre-replication complex (pre-RC)” is formed on each origin. The pre-RC contains several proteins including the origin recognition complex (ORC), Cdc6p, Cdt1p, and the six minichromosome maintenance proteins (MCM), Mcm2–7p. The ORC was originally identified as a six-protein complex that specifically bound to S. cerevisiae origins of DNA replication (5), and its homologues have been found in various eukaryotic species, including human (3). In this manuscript, “ORC” refers to S. cerevisiae ORC. The ORC is bound to chromatin at the origins of chromosomal DNA replication throughout the cell cycle and is thought to function as a “landing pad” for the assembly of pre-RC. At the G1-S boundary, CDK and another kinase (Cdc7p-Dbf4p) activate the pre-RC to initiate chromosomal DNA replication.
Source: http://www.jbc.org/content/284/6/3396.long