H+ concentration gradient Photosynthesis

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SUMMARY

The H+ concentration gradient across the thylakoid membrane during photosynthesis is maintained through three primary mechanisms: photolysis, electron transport from photosystem II via the cytochrome b6/f complex, and the formation of NADPH. The cytochrome b6/f complex actively pumps H+ ions into the thylakoid lumen during electron transport, creating a proton gradient essential for ATP synthesis. This process is crucial as ATP synthase requires a proton gradient to generate ATP, highlighting the necessity of this multi-step energy transfer mechanism in photosynthesis.

PREREQUISITES
  • Understanding of photosynthesis, specifically light-dependent reactions
  • Familiarity with the role of the cytochrome b6/f complex
  • Knowledge of ATP synthase function and proton gradients
  • Basic concepts of electron transport chains in chloroplasts
NEXT STEPS
  • Study the role of the cytochrome b6/f complex in detail
  • Explore the mechanism of ATP synthesis via ATP synthase
  • Investigate the process of photolysis in photosynthesis
  • Learn about the electron transport chain in chloroplasts
USEFUL FOR

Students of biology, particularly those studying plant physiology, educators teaching photosynthesis, and researchers focusing on energy transfer mechanisms in photosynthesis.

garytse86
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FOr the light dependent reaction, In the textbook it says that the H+ concentration gradient across the thylakoid membrane is maintained by:

1) photolysis
2) transport of electrons from photosystem II along carriers
3) formation of NADPH.

I can understand 1) and 3), but I have no idea how transport of e- would maintain a hydrogen ion concentration gradient. Can someone help please?

THanks.

Gary
 
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Does this statement help:

The cytochrome b6/f complex acts to pump H+ across the thylakoid during electron transport
 
so this is exactly the same as cyclic phosphorylation then? energy from e- is used to pump H+ across thylakoid membrane?
 
but it seems silly for plants to have evolved such mechanisms.
Why pump the hydrogen into the lumen, and when they diffuse out along the electrochemical gradient the energy is used to synthesise ATP. Why go through this process, why can't the energy just be used to synthesise ATP in the first place?
 
Energy for e- is used to pump H+ out. ATP is synthesis due to that process, which is why H+ are pump out.

Look at the figure
http://bio.winona.msus.edu/bates/Bio241/images/figure-08-12-1.jpg
 
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why go through H+? why can't the energy from the electrons be directly used to synthesise ATP?
 
There is no mechanism to do it directly. ATP synthase is powered by the proton gradient. The only way to make ATP is to use the enzyme ATP synthase, because of the structure of the ATP. Energy has to be transferred from photons to electrons to the H+ gradient, then to ATP.
 
nipwoni said:
There is no mechanism to do it directly. ATP synthase is powered by the proton gradient. The only way to make ATP is to use the enzyme ATP synthase, because of the structure of the ATP. Energy has to be transferred from photons to electrons to the H+ gradient, then to ATP.
oh now i get it. It is because of the structure of the enzyme which needs a H+ gradient.


Many thanks to all of you.

Gary
 

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