The discussion centers on the need for primers for porcine bcl-xs to conduct RT-PCR for gene expression analysis in swine. Users recommend utilizing the National Center for Biotechnology Information (NCBI) for gene sequence searches, although it is noted that a complete gene sequence for bcl-xs is currently unavailable. Participants suggest that users may need to design their own primers using various free online tools, as specific guidelines for RT-PCR primer design are not provided in the discussion.
PREREQUISITESResearchers in molecular biology, swine geneticists, and anyone involved in gene expression studies using RT-PCR techniques.
Ygggdrasil said:The National Center for Biotechnology Information, which curates many sequence databases, would be a good place to search:
http://www.ncbi.nlm.nih.gov/nucleotide/
For primers, you would likely have to design these yourself. There are many free programs available on the web to assist in PCR primer design (I've never done RT-PCR, so I don't know the specifics of primer design for that particular application).[/QUOt
The problem is that there is no full gene sequence of Bcl-xs.