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Help ~I need the primers of porcine bcl-xs

  1. Jun 7, 2010 #1
    help~~~~~I need the primers of porcine bcl-xs

    I want to use RT-PCR to test the expression change of bcl-xs on swine. But I can't find the gene sequnece or primers. I will sincerely appreciate your help.
     
  2. jcsd
  3. Jun 7, 2010 #2

    Ygggdrasil

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    Re: help~~~~~I need the primers of porcine bcl-xs

    The National Center for Biotechnology Information, which curates many sequence databases, would be a good place to search:
    http://www.ncbi.nlm.nih.gov/nucleotide/

    For primers, you would likely have to design these yourself. There are many free programs available on the web to assist in PCR primer design (I've never done RT-PCR, so I don't know the specifics of primer design for that particular application).
     
  4. Jun 8, 2010 #3
    Re: help~~~~~I need the primers of porcine bcl-xs

     
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