Hello everyone, I'm very new to microbiology and does not have any background knowledge, so I have some simple questions on identifying different bacteria on agar plates. I have some questions and I have answered them the way I think. Please correct me where nessecary. 1. First of all what do all the lines in that plate mean, and how do you make a culture plate? Ok so you get the agar medium, and dip a bacterial loop into your sample eg CSF and then streak them on agar plate. So I'm thinking those lines are bacterial streaks. 2. Now how do I know there is growth in the culture plate and what is the difference between streaks and circles in the above pic? Do the circles mean bacterial colonies , then what do the streaks mean?I would like to see an agar plate where there is no bacterial growth, how does that agar plate look like, does it still have original streaks, or would it just be the agar without any streaks. 3. Ok this is a picture of proper streaking and growth of H. influenzae on a CAP. Now how do I differentiate this bacteria from other bacteria capable of growing on choclate agar. Also my orginal pic is proper streaking and growth of N. meningitidis on a BAP, same question prevails for this. 4. Are bacterias able to grow on all agar types or do they have specific ones. If anyone can answer thanks a lot in advance I'll post more pics depending on the replies.