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Thread moved from the technical forums to the schoolwork forums
TL;DR Summary: I have to do a project for my school, and I want to know how it works, because I just can't get any useful information of the internat alone.
I have a project for school, I want to analyse and compare the protein contents of some different species of fish. The problem I'm stuck with, is just separating the protein from nucleic acids and other organic matter. I want to get the concentration by either using the Biuret test, or using Ninhydrin so that I can use a photometer to get that concentration. But still I am either stuck with lysing my sample, and getting that protein, especially with the regulations we have at my school, I cannot use hard chemicals e.g. I can't do a phenol-Chloroform extractionl.
I have a project for school, I want to analyse and compare the protein contents of some different species of fish. The problem I'm stuck with, is just separating the protein from nucleic acids and other organic matter. I want to get the concentration by either using the Biuret test, or using Ninhydrin so that I can use a photometer to get that concentration. But still I am either stuck with lysing my sample, and getting that protein, especially with the regulations we have at my school, I cannot use hard chemicals e.g. I can't do a phenol-Chloroform extractionl.