How does genetic linkage affect testcross and recombination frequency?

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SUMMARY

This discussion clarifies the relationship between genetic linkage, testcrosses, and recombination frequency. Genetic linkage occurs when two genes are located close together on the same chromosome, leading to a lower frequency of recombination between them. Conversely, genes that are further apart have a higher likelihood of being separated during recombination events. The physical distance between genes, measured in base pairs, directly influences the probability of recombination, impacting genetic inheritance patterns.

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  • Understanding of genetic linkage and its implications
  • Familiarity with testcross methodology
  • Knowledge of recombination frequency calculations
  • Basic genetics terminology, including chromosomes and base pairs
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  • Study the principles of testcrosses in genetic analysis
  • Explore the impact of physical distance on recombination frequency
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Ukitake Jyuushirou
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as the title suggest, i am confused when i read about "testcross" and linkage
 
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Genetic linkage means that two genes segregate together, which means that they are close together on the same chromosome.

The measure of linkage is the frequency at which two genes become separated during recombination, when they are further apart it is more likely that they become separated.
 
Just to expand on what has already been said.

If genes are linked then they act as if they are on the same chromosome. That is to say whenever gene A is observed so is gene B. However, for non-sex chromosomes, each person has 2 of the "same" autosome, one from the maternal line and the other from the paternal line. There is a chance that recombination will take place between these the paternal and maternal autosome thereby making a hybrid paternal and maternal chromsome. If the genes are physically far apart on the chromsome there is a higher frequency that they will undergo recombination (the shuffling stuff that makes the hybrid chromosome.) If the genes are physically really close, then there is a lower frequency of recombination.

By physically I mean literally mean physically, i.e. number of base pairs apart.
 

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