How Does pH Affect Amylase Activity?

[GaMeR4LyF]

Hi guys, i have been set some coursework by school on the investigation of the effect of pH on the enzyme amylase. I am abit lost right now only just started A levels. Can someone suggest how i would carry out this investigation? Equipment and method would be much appreciated! And the results need to be quantitative and not qualitative.

 

[GaMeR4LyF]

ahh my bad wrong board

 

[Wild Angel]

well you could use your spit (contains the enzyme) , pieces of bread (substrate) ,lemon juice/vinegar (to provide acidic medium) ,water (ph7.7 preferably distilled water),and an alkaline solution.To test to see if glucose has been produced use an iodine solution.
Blue-black colour indicates that the result is positive.equipment needed : petri dishs

 

[GaMeR4LyF]

hmmm...problem with iodine is that it doesn't give accurate enough readings to differenciate between the samples

 

[Wild Angel]

Try using a stronger acid and a stronger base. Remember salivary amylase works best in a range of +/- 6.0 - 8.0 .