How Does pH Affect Amylase Activity?

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The discussion focuses on investigating how pH affects amylase activity, particularly for a school coursework project. Participants suggest using saliva as a source of amylase, bread as the substrate, and various acids and bases to create different pH environments. Iodine solution is recommended for testing glucose production, with a blue-black color indicating a positive result. However, there are concerns about the accuracy of iodine readings, and suggestions are made to use stronger acids and bases for better differentiation. Salivary amylase is noted to function optimally within a pH range of 6.0 to 8.0.
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Hi guys, i have been set some coursework by school on the investigation of the effect of pH on the enzyme amylase. I am abit lost right now only just started A levels. Can someone suggest how i would carry out this investigation? Equipment and method would be much appreciated! And the results need to be quantitative and not qualitative.
 
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well you could use your spit (contains the enzyme) , pieces of bread (substrate) ,lemon juice/vinegar (to provide acidic medium) ,water (ph7.7 preferably distilled water),and an alkaline solution.To test to see if glucose has been produced use an iodine solution.
Blue-black colour indicates that the result is positive.equipment needed : petri dishs :smile:
 
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hmmm...problem with iodine is that it doesn't give accurate enough readings to differenciate between the samples
 
Try using a stronger acid and a stronger base. Remember salivary amylase works best in a range of +/- 6.0 - 8.0 .:smile:
 

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