I was reading an article on Pseudomonas syringae and how removing a gene called inaZ from it's DNA will allow it to be sprayed on crops to prevent the crops from freezing during the winter. How is the inaZ gene removed from the DNA? I'm thinking that they use restriction enzymes that cut out the specific sequence of the gene, and then ...I'm not sure how the DNA is put back together. Does a DNA ligase just come around and bind the two ends together? What restriction enzyme is used on Pseudomonas syringae dna? Please correct me, thanks!