How to Determine Ka of Bromcresol Green from Absorbance Values?

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Discussion Overview

The discussion revolves around determining the acid dissociation constant (Ka) of bromcresol green using absorbance values from various solutions. Participants are exploring the interpretation of a specific equation related to absorbance and its application in calculating Ka, as well as the experimental setup involving multiple solutions.

Discussion Character

  • Homework-related
  • Technical explanation
  • Debate/contested

Main Points Raised

  • One participant seeks clarification on the equation involving absorbance values, questioning whether it involves subtraction or multiplication by negative values.
  • Another participant points out that Ka should be constant for a given substance, suggesting that the term "Ka for 5 different solutions" may refer to experimental values rather than distinct constants.
  • A participant describes the experimental procedure, detailing the preparation of solutions and the method of measuring absorbance to find maximum values.
  • There is uncertainty about the meaning of the variable An, with one participant providing a definition as absorbance at maximum wavelength for the negative ion form.
  • Some participants suggest that the Henderson-Hasselbalch equation may be relevant for calculating pKa and Ka based on pH and absorbance ratios.
  • One participant expresses confusion about how the absorbance values relate to the concentrations of bromcresol green in its acid and conjugate base forms.

Areas of Agreement / Disagreement

Participants express differing views on the interpretation of the equation and the meaning of specific variables. There is no consensus on how to proceed with the calculations or the relationship between absorbance and concentrations.

Contextual Notes

Participants highlight the need for clarity on variable definitions and the relationship between absorbance and concentration, indicating potential missing assumptions or definitions that could impact the discussion.

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Homework Statement



I need to find out the ka of bromcresol green for a 5 different solutions. I have the absorbance at the maximum wavelength for each solution. In the lab report the next box says

An-Ahb / Ab(negative ion) -An

i have the values for An,Ahb, and Ab but I am not sure if the equation is subtracting the values or multiplying by negative values

Homework Equations





The Attempt at a Solution

 
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Please elaborate - what is what of what?
 
Last edited by a moderator:
im not sure what your saying, what other information is needed.
 
What do you mean - Ka for 5 different solutions? Ka is constant for a given substance, so it should be identical for all solutions. I guess you mean "experimental value of Ka for each experiment".

What is An? I guess Ahb is meant to be AHB, but you can't assume every symbol is obvious to everyone.

I guess your post means that you should now calculate value of the expression and you are not sure how to interpret this expression. I have the same problem with your post - it is not much more precise than the expression you posted :wink:
 
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ill give you an overview of the lab.

we had to make 8 solutions. solution A then solutions 1-7. solution A had 25.00ml of HOAc dilluted to 100ml with water. this solution was but into a buret.

solution one has 5.00ml of bromcresol green(BCG) and 5.00ml NaOAc, diluted to 100ml.

we then took absorbance readings of solution 1 with a spectrophotometer to find out the maximum absorbance.

solution 2 then got 2.00ml of solution A added to it with the buret. Solution three got 2.00ml of solution A(for a total of 4.00ml) and so on till solution 7(which had 2.00ml of solution one added to it for a total of 12.00ml).

all solutions were made from the previous one and each solution was measured at the maximum absorbance except for 3 and 7 which were measured for the full scale of wavelengths to figure out there maximum absorbance readings.
 
Still no idea what is An.

I guess your calculations should be based on Henderson-Hasselbalch equation. You know pH (you forced it using acetate buffer), you have absorbances that can be used to calculate ratio of BCG both in the form of acid and conjugate base - that's enough to calculate pKa and Ka.
 
An = absorbance at maximum wavelength B-

[H30+] = Ka[HOAc/[OAc-]
 
I would concentrate on trying to understand whether and if - how
An-Ahb
and
Ab(negative ion) -An
are related to concentrations of BCG in the form of acid and conjugate base.

But I think I have already signaled it.
 
Last edited by a moderator:

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