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How to determine zinc in a plant.

  1. Feb 11, 2012 #1
    Which would you prefer? I'm calculating the amount of zinc absorbed by a particular plant (yes, in theory it does accumulate zinc- B.Juncea).
     
  2. jcsd
  3. Feb 11, 2012 #2

    Borek

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    Staff: Mentor

    Re: AAS or Calibration curve method to determine zinc in a plant.

    Question doesn't make sense. How are you going to use AAS without a calibration curve?
     
  4. Feb 11, 2012 #3
    Re: AAS or Calibration curve method to determine zinc in a plant.

    Woops! ignore my ignorance, I was just told that, well doesn't the AAS give you the concentration directly? Gosh, just pointless maches *argh*

    CHANGE: AAS or COLORIMETER
     
  5. Feb 12, 2012 #4
    How would you extract zinc from a plant?

    Hi!

    So I basically need to find out how I'm supposed to extract zinc from a plant and its methodology and chemicals required. How do I do this? I found one article that is rather incomplete, it just says zinc was extracted using 0.1N HCl and not whether the plant's powder was treated with it or the entire plant was treated with it.

    Here's a "www.sc.chula.ac.th/nrc-ehwm-fs/research/journal/5.pdf" [Broken].
     
    Last edited by a moderator: May 5, 2017
  6. Feb 12, 2012 #5
    Your reference has some clues (there are citations about preparing samples of soil). Start with these and work through references that will be found in those initial citations. Literature research always gives clues about how to proceed and achieve reproducibility in any analysis.

    Another reference (found in many science libraries) would be the A.O.A.C Methods which are matrix specific analyses for foodstuffs, crops, soil, and other agricultural i.e. They can be somewhat difficult to navigate but official methods can be found for plant and soil analysis.

    Official Methods of Analysis of AOAC INTERNATIONAL,18th Edition

    Wiki AOAC International...
     
  7. Feb 13, 2012 #6
    Urgent: Some complexing agents for zinc.

    Hi, I am to extract Zinc from plant tissue, with 0.1N HCl. But since its colorless I cannot determine the concentration(using the colorimeter) of zinc without adding a chemical that forms a complex with zinc and has a color.
     
  8. Feb 14, 2012 #7

    Borek

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    Staff: Mentor

    Have you followed MrSid's hints?
     
  9. Feb 14, 2012 #8
    Sorry, that's beyond me! :O I'm just a 17 year old guy! Please sympathize with me, this is tough enough with so much chemistry that's waay about my level!
     
  10. Feb 14, 2012 #9

    DrDu

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    I suppose then that you don't have an atomic absorption spectrometer at hand but only a colorimeter.
    Before determining the concentration of Zn usin colorimetry, you will have to separate it from other elements. That's all rather complicated.
    I found an old article describing how this can be done and which in principle can be done in the lab of a school. However, it is in German and the method requires a lot of work.

    http://onlinelibrary.wiley.com/doi/10.1002/jpln.19560740105/pdf
     
  11. Feb 14, 2012 #10
    Talk about luck haha! My mum's been teaching german for 15 years, however, A complexing agent I did come across was zincon, which was holy costly!! Yes, so sorry for the late up date. But I'm going with AAS since I can't find a complexing agent other than zincon for zinc.
     
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