How to insert genes in a plant?

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Discussion Overview

The discussion revolves around the methods and processes involved in inserting genes from one plant species into another. Participants explore various techniques, the necessary background knowledge, and the practicalities of genetic engineering in plants.

Discussion Character

  • Exploratory
  • Technical explanation
  • Debate/contested
  • Homework-related

Main Points Raised

  • One participant inquires about the process of inserting genes from one plant species into another, expressing a desire to create new plants.
  • Another participant suggests that studying biology and joining a relevant working group is essential, emphasizing that plants cannot be treated like simple building blocks for genetic modification.
  • A participant shares their experience with viral vectors in C. elegans, indicating a parallel to potential methods in plants.
  • Another participant mentions the use of bacteriophages as viral vectors with bacteria, noting a lack of experience with plants but referencing methods like gene guns and bacterial integration.
  • One participant describes the use of a gene gun to insert plasmids into plant material, highlighting the need for preparation of seeds and questioning the original poster's background in plant biology and genetics.
  • There is a call for the original poster to clarify their goals and available resources to receive more tailored advice.

Areas of Agreement / Disagreement

Participants express varying opinions on the feasibility and methods for gene insertion in plants, with no consensus reached on a specific approach or technique. The discussion remains unresolved regarding the best practices and necessary qualifications for such genetic engineering tasks.

Contextual Notes

Limitations include the lack of detailed information on specific methods, the need for background knowledge in genetics, and the practical requirements for conducting genetic engineering in plants.

Richard_Steele
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How can I insert pieces of other plant genes into another different specie of plant.
I want to create new plants, so I would know the process.
 
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The easiest way: Study biology, look for a working group doing something similar and join them. Long before you have the required knowledge to do this, you'll know several methods to transfer genes.
Plants are not lego, you cannot just put a random gene of one plant into another and get something interesting new.
 
When I worked in an experimental lab, we used viral vectors with C. elegans.
 
mfb said:
The easiest way: Study biology, look for a working group doing something similar and join them. Long before you have the required knowledge to do this, you'll know several methods to transfer genes.
Plants are not lego, you cannot just put a random gene of one plant into another and get something interesting new.

You know any group on the net that are involved in such task?
I've asked to some people near of me and no one is involved in any similar project, so I've to check the internet to find it.
 
Pythagorean said:
When I worked in an experimental lab, we used viral vectors with C. elegans.

Similar thing here except we used bacteriophages as viral vectors with bacteria.

I've never worked with plants before but a quick glance at Wikipedia tells me that they use gene guns that directly inject the genetic material through the cell wall in the form of metal particles coated with plasmid DNA, as well as a completely different process involving a species of bacterium that is able to integrate its plasmid DNA into a plant host.
 
You need a gene gun to insert plasmids into plant material, IIRC you have to prepare the seeds to be more receptive (this involves removing any outer shells) before firing plasmid loaded particles into them.

What background do you have in plant biology and genetics? Do you know how to design and construct plasmids? Do you have access to relevant equipment? If not then I advise that you seek out education and employment in this field as mfb has said.

Either way it would be helpful if you could provide information as to what your goal is and what means you have to do it so people can help you properly rather than having to guess whether to pitch their answers towards an amateur aiming to do work in their garage or a PhD student wanting to cross disciplines.
 

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