How would i go about breaking an isopeptide bond?

[okkvlt]

Hi. i don't know a lot about chemistry so bear with me.
the bond is between a member of the carboxyl group and the amino acid lysine.
as i understand i need an enzyme. what readily available enzyme would suffice?
would protease work?

 

[chemisttree]

Your question doesn't make sense as presented. The peptide bond is between the amino functionality of an amino acid (lysine?) and the carboxy functionality of a different amino acid (also lysine?). The proteases as a class would be the enzyme you are looking for but HCl/heat would work as well. If you are looking for a lysine (lys) specific protease at the end of a peptide, http://www.roche-applied-science.com/pack-insert/1420429a.pdf" would work.

 

[okkvlt]

nevermind. i got what i want. trypsin from the local health mart.