Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

How would i go about breaking an isopeptide bond?

  1. Jan 5, 2010 #1
    Hi. i dont know alot about chemistry so bear with me.
    the bond is between a member of the carboxyl group and the amino acid lysine.
    as i understand i need an enzyme. what readily available enzyme would suffice?
    would protease work?
    Last edited: Jan 5, 2010
  2. jcsd
  3. Jan 5, 2010 #2


    User Avatar
    Science Advisor
    Homework Helper
    Gold Member

    Your question doesn't make sense as presented. The peptide bond is between the amino functionality of an amino acid (lysine?) and the carboxy functionality of a different amino acid (also lysine?). The proteases as a class would be the enzyme you are looking for but HCl/heat would work as well. If you are looking for a lysine (lys) specific protease at the end of a peptide, http://www.roche-applied-science.com/pack-insert/1420429a.pdf" [Broken]would work.
    Last edited by a moderator: May 4, 2017
  4. Jan 7, 2010 #3
    nevermind. i got what i want. trypsin from the local health mart.
Share this great discussion with others via Reddit, Google+, Twitter, or Facebook