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How would i go about breaking an isopeptide bond?

  1. Jan 5, 2010 #1
    Hi. i dont know alot about chemistry so bear with me.
    the bond is between a member of the carboxyl group and the amino acid lysine.
    as i understand i need an enzyme. what readily available enzyme would suffice?
    would protease work?
     
    Last edited: Jan 5, 2010
  2. jcsd
  3. Jan 5, 2010 #2

    chemisttree

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    Your question doesn't make sense as presented. The peptide bond is between the amino functionality of an amino acid (lysine?) and the carboxy functionality of a different amino acid (also lysine?). The proteases as a class would be the enzyme you are looking for but HCl/heat would work as well. If you are looking for a lysine (lys) specific protease at the end of a peptide, http://www.roche-applied-science.com/pack-insert/1420429a.pdf" [Broken]would work.
     
    Last edited by a moderator: May 4, 2017
  4. Jan 7, 2010 #3
    nevermind. i got what i want. trypsin from the local health mart.
     
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