It's possible to ignite gasoline with a cigarette, but just barely.
http://mythbustersresults.com/special7
Ignition temperature for various fluids
Gasoline: 800 deg
Propane: 871 deg
Flour: 748 deg
Acetylene gas: 571 deg
60 Octane gas: 536 deg
Paper: 446 deg
#1 Fuel oil: 444 deg
Wood: 392 deg
Cigarette temperature when inhaling: about 550 deg
There's also a small possibility of vapors accumulating and igniting (vapors have a lower ignition temperature). While you could accumulate vapors in a gas can, it's very unlikely that enough vapors would accumulate in an open air gas station while refueling your car - especially if there's any kind of breeze.
It's not particularly easy to drop a lit cigarette into your gas tank nowadays, especially if you've got the nozzle stuck in the refueling tube, so, while it's possible the vapors in your car's gas tank could be ignited, the odds aren't very good.