SUMMARY
The correct acid dissociation constant (Ka) for Phosphate Buffered Saline (PBS) at pH 7.4 is 1.59E-7, as derived using the Henderson-Hasselbalch equation with the concentrations of 0.008M Na2HPO4 and 0.002M KH2PO4. While some sources cite a Ka of 6.2E-8, the calculations based on the provided pH and pKa values (2.148, 7.199, 12.35) confirm the higher value. It is essential to consider the ionic strength of the solution when performing these calculations to ensure accuracy.
PREREQUISITES
- Understanding of the Henderson-Hasselbalch equation
- Knowledge of pKa values and their significance in buffer solutions
- Familiarity with the composition of Phosphate Buffered Saline
- Basic principles of ionic strength and its effect on pH calculations
NEXT STEPS
- Study the Henderson-Hasselbalch equation in detail
- Research the effects of ionic strength on buffer solutions
- Explore the significance of pKa values in biochemical applications
- Learn about the preparation and use of Phosphate Buffered Saline in laboratory settings
USEFUL FOR
Biochemists, laboratory technicians, and researchers working with buffer solutions and pH calculations will benefit from this discussion.