MCQ: Enzyme Kinetics - Find the Zeroth Order Answer

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SUMMARY

An enzyme-catalyzed reaction becomes zeroth order when the substrate concentration significantly exceeds the Michaelis constant (Km), specifically when [S] >> Km. In this scenario, the reaction velocity reaches its maximum (Vmax) and is independent of substrate concentration. The correct answer to the multiple-choice question is option b, indicating that the velocity of enzyme action is very high. This conclusion is based on the understanding that zeroth-order kinetics occur under conditions where substrate concentration is not limiting.

PREREQUISITES
  • Understanding of enzyme kinetics and reaction orders
  • Familiarity with the Michaelis-Menten equation
  • Knowledge of substrate concentration and its effect on enzyme activity
  • Basic principles of enzyme catalysis
NEXT STEPS
  • Study the Michaelis-Menten equation in detail
  • Learn about enzyme inhibition and its effects on reaction kinetics
  • Explore the differences between zero-order and first-order kinetics
  • Investigate practical applications of enzyme kinetics in biochemical assays
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Students studying biochemistry, researchers in enzymology, and professionals involved in biochemical assay development will benefit from this discussion.

Tyto alba
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Homework Statement


An enzyme catalysed reaction becomes zeroth order when:

a.The velocity of enzyme action very low
b. The velocity of enzyme action very high
c.Enzyme conc very high
d. All of these The attempt at a solution
I understand An enzyme mediation recation becomes Zeroth order if [ S]>>Km where Km= [ S] when Vo=Vmax/2.I really have no idea what way I should approach this MCQ.
 
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Tyto alba said:

Homework Statement


An enzyme catalysed reaction becomes zeroth order when:

a.The velocity of enzyme action very low
b. The velocity of enzyme action very high
c.Enzyme conc very high
d. All of these The attempt at a solution
I understand An enzyme mediation recation becomes Zeroth order if [ S]>>Km where Km= [ S] when Vo=Vmax/2.I really have no idea what way I should approach this MCQ.

Well unless there is excess substrate inhibition, then for any given concentration of enzyme the reaction velocity is as high as it can be when [ S]>>Km, and I think you should answer b and not worry about it because you are getting some rather bad questions. The zero order is related strictly not to the velocity, but to the substrate concentration as you realize.
 
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