Measurement of concentration of urea

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Discussion Overview

The discussion revolves around methods for determining the concentration of liquid urea, specifically in the range of 0.5-1.5 mol/L. Participants explore various laboratory techniques and approaches for measuring urea concentration in samples, particularly those obtained from sandy soil that may contain impurities.

Discussion Character

  • Exploratory
  • Technical explanation
  • Debate/contested
  • Experimental/applied

Main Points Raised

  • One participant inquires about laboratory techniques or kits for measuring urea concentration in liquid samples, emphasizing the need for measurements in mol/L.
  • Another participant suggests diluting the sample and using spectrophotometric determination with diacetyl monoxime, followed by calculations to find the original concentration.
  • A participant questions the effectiveness of the suggested method in the presence of impurities, describing a specific experimental setup involving injecting urea into a tube filled with sand and collecting samples from the other end.
  • One participant expresses confusion regarding the initial inquiry, prompting a clarification about the context of the water samples and their turbidity, along with the desire to isolate urea concentration.
  • The same participant considers the possibility of using enzymes to dissociate urea but expresses doubt about the quantitative nature of this approach.
  • A later reply references a paper and its associated references, implying that further reading may provide additional insights.

Areas of Agreement / Disagreement

Participants do not reach a consensus on the best method for measuring urea concentration, with multiple competing views and uncertainties regarding the impact of impurities and the effectiveness of suggested techniques.

Contextual Notes

Participants mention potential challenges such as sample turbidity and the presence of impurities, which may affect measurement accuracy. There is also uncertainty regarding the quantitative reliability of using enzymes for dissociating urea.

duke656
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Dear All

Does anyone have any idea about how to determine the concentration of liquid urea. The concentration is a bit higher (0.5-1.5 mol/L). Is there any laboratory techniques or Kit? I looked for and found everywhere the method to measure the amount of urea in blood and urea but what I want is in terms of mol/L.

Thanks in advance.
 
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You can always dilute your sample, use one of the methods for which the procedure is easy to to obtain (perhaps spectrophotometric determination with diacetyl monoxime?), then calculate the concentration in the original sample.
 
Thank you Borek.

Will this method work if there is some impurities other than urea? For example I will inject 0.5 M urea into one end of a 1 m long tube full of sand. Then I collect samples from the other end and try to evaluate the concentration.

Thanks in advance.

Peace
Deb
 
duke656 said:
Dear All

Does anyone have any idea about how to determine the concentration of liquid urea. The concentration is a bit higher (0.5-1.5 mol/L). Is there any laboratory techniques or Kit? I looked for and found everywhere the method to measure the amount of urea in blood and urea but what I want is in terms of mol/L.

Thanks in advance.

You found the method everywhere and you want to know what it is? :confused: Hard to understand what your problem is.
 
Probably, I could not make you understand. Let me start from the beginning. I have some water samples obtained from the sandy soil. They are a bit turbid and contain urea in higher concentrations (more than 0.5 mol/L). I would like to evaluate the concentrations of only urea of each of these samples. Please share if you know any easier method. Do you suggest me to dissociate it using some enzymes? But it does not sound quantitative is it?
 
I assume you've read and are familiar with this paper and the references within? Perhaps this one as well?
 

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