[tex]\Delta[/tex]9-Tetrahydrocannabinol (THC) is the primary psychoactive constituent of marijuana. Its metabolites include 11-hydroxy- [tex]\Delta[/tex]9-THC (11-OH-THC) and 11-nor-9-carboxy- [tex]\Delta[/tex]9-THC (THC-COOH).
1)Write down the molecular structure of these three compounds.
2)Develop an analytical method to determine ng/mL levels of THC, 11-OH-THC and THC-COOH in urine samples, which include:
a)step-by-step sample preparation
Please provide your reasoning for choosing a specific method or instrument.
The Attempt at a Solution
Well, I looked up the structure of THC, but as for the notation used for the description of the structure, I'm not sure what they all mean. It's been awhile since I took organic...
Anyway, for the structures, Is it correct to say that it's the ones I found in the links?
1) THC structure: http://en.wikipedia.org/wiki/Tetrahydrocannabinol" [Broken]
11-hydroxy- [tex]\Delta[/tex]9-THC (11-OH-THC) : http://en.wikipedia.org/wiki/11-Hydroxy-THC
11-nor-9-carboxy- [tex]\Delta[/tex]9-THC (THC-COOH) : http://en.wikipedia.org/wiki/11-nor-9-Carboxy-THC" [Broken]
What does the, "nor" and the [tex]\Delta[/tex] stand for in the notations?
2) I guess I can look up the steps and such, but with calibration, how would that work?
Would there be an external standard for the instrument (LC-MS or other instrument)
In addition I hear about derivatization of samples prior to analysis for this in some papers. What is derivatization and why is it needed here?
Thank you and the last question I bolded is something I need help on.
Last edited by a moderator: