Hello, I am reading about the Western blot technique, and I am looking for clarification of the transfer step described in the wikipedia article and in this video walkthrough. It is not clear to me why the proteins should keep their shape needed for the probing step rather than breaking or deforming. Is this because the strength of bonds within the proteins are stronger than the electrical force pulling them to the nitrocellulose membrane? Or is the whole protein pulled uniformly? My knowledge of physics involved in protein structure is virtually null.