Molecular Weight for nucleotides

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SUMMARY

The discussion clarifies the concept of molecular weight for nucleotides, specifically addressing the meaning of "daltons" and the notation "(g/M)". A dalton (Da) is defined as an atomic mass unit, with C12 having a mass of exactly 12.0000 Da. The notation (g/M) stands for grams per mole (g/mol), indicating the mass of one mole of a substance. The conversation emphasizes that the molecular weight of carbon is used as a reference point due to its ease of purification, establishing a standard for measuring molecular weights of other elements.

PREREQUISITES
  • Understanding of atomic mass units (daltons)
  • Basic knowledge of moles and molar mass
  • Familiarity with molecular weight calculations
  • Concept of grams per mole (g/mol)
NEXT STEPS
  • Research the molecular weight of various nucleotides
  • Learn about the significance of atomic mass in biochemical contexts
  • Explore the process of purifying elements for accurate molecular weight determination
  • Study the relationship between molecular weight and chemical reactivity
USEFUL FOR

Biochemists, molecular biologists, and students studying molecular biology who need to understand the principles of molecular weight and its applications in nucleotide analysis.

mountain
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When it says; "Molecular Weight: daltons (g/M)" What does daltons mean and what does (g/M) stand for? Does g/M stand for g/mol or what?

How can they calculate Molecular Weight for nucleotides?


Thanks for any inputs.
 
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A dalton is an atomic mass. For instance C12 has a mass of 12.0000 Da. The mw is the mass of 1 mole of a particular molecule. By definition 1 mole of carbon atoms weighs 12 grams.
 
Thanks.

But what does M stand for in g/M?
 
mountain said:
Thanks.

But what does M stand for in g/M?

You had it right...mole.
 
Monique said:
A dalton is an atomic mass. For instance C12 has a mass of 12.0000 Da. The mw is the mass of 1 mole of a particular molecule. By definition 1 mole of carbon atoms weighs 12 grams.
(why only four zeros?)

(1) C12 has a mass of 12 Da (by definition; exactly 12, there is no uncertainty)
(2) a mol of C12 atoms weighs 12 grams (by definition)

From (1) and (2) it follows that a dalton is the number of grams that go into a mol (gram/mol)
__________________________________________________
(1) C12 = 12 Da <--> mol C12 = mol 12 Da
(2) mol C12 = 12 gram
(substitute 2 into 1) 12 gram = mol 12 Da <--> gram/mol = Da
 
doesn't g/M mean g/mol/L?
 
its not exactly 12, and its not exactly 16 for oxygen either

but they decided to make it 12 anywho. so just use 12 as a whole number

6 protons = 6 x 1.007277 6.043662
6 neutrons = 6 x 1.008665 6.051990
6 electrons = 6 x 0.000548 0.003288
Total 12.098940
 
Last edited:
Carbon was easy to purify, that is why they used it as a reference and why it is the whole number 12.
 

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