Needing to calculate kDa for Hyaluronic acid

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Discussion Overview

The discussion revolves around calculating the kDa (kilodalton) value for Hyaluronic acid based on provided values from a fact sheet. Participants explore the relationship between molecular weight, density, and viscosity in the context of Hyaluronic acid, which is relevant to chemistry and biochemistry.

Discussion Character

  • Exploratory
  • Technical explanation
  • Debate/contested

Main Points Raised

  • One participant expresses uncertainty about calculating kDa from the given values and requests assistance.
  • Another participant explains that kDa is a unit of molecular weight, equating 1 kDa to 1000 Da.
  • A different participant provides a calculation based on the assumption that 1.14M refers to 1.14 million, suggesting a kDa value of approximately 1140 kDa.
  • One participant clarifies their understanding of low molecular weight Hyaluronic acid, indicating that if 1.14 represents kg/mol, the kDa would be 114 kDa, while if it is in g/mol, it would be 1.14 kDa, which they consider too low.
  • Another participant requests the source of the numbers to verify the information provided.

Areas of Agreement / Disagreement

Participants do not reach a consensus on the interpretation of the values provided or the correct calculation of kDa, indicating multiple competing views and uncertainty regarding the definitions and measurements involved.

Contextual Notes

There is ambiguity regarding the units of the molecular weight provided (1.14M) and whether it refers to molarity or mass, which affects the calculation of kDa. Additionally, the implications of kDa values on inflammation are mentioned but not resolved.

Tarn
Please post this type of questions in HW section using the template and showing your work.
I know very little about chemistry so not sure if this is possible. I am wanting to find out the kDa from the values given in the fact sheet ( I'm not sure exactly what it can be calculated from but these are the values from the fact sheet )
molecular weight 1.14M
bulk density .34g/cm3
limiting viscosity 18.8 dl/g
Just wondering if anyone can calculate the kDa for me as I've spent the last 2 hours trying to work out how to do it but I'm lost!
Thanks :smile:
 
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kDa stands for kilodalton. It's a unit of molecular weight. More precisely, 1 dalton = 1g/mol, and 1 kDa = 1000 Da.
 
kDa is measure of molecular weight. One hydrogen atom mass == 1 Da. Proteins and other macromolecule molecular weights are usually measured in 1000 Da or kDa.

Hyaluronic acid is a polymer: (C14H21NO11)n where n can be a large number. So you have 1.14M, I'm taking that to mean 1.14 million. So divide 1.14 million by 1000 to get kDa. Answer is ~= 1140 kDa.

Edit -- TeethWhitener beat me to it.
 
Thanks for your help. I'm still a bit lost as I have low molecular weight Hyaluronic so it shouldn't be over 1000. But I'm leaning towards M meaning Molar mass which is what it seems to represent which is often measured in g/mol but standard measurement is kg/mol. So if it is 1.14 kg/mol and 1 kDa is equal to 1g/mol then I'm thinking it's 114 kDa which is considered to be in the low range so perfect. If the 1.14 is measured in g/mol then it would be 1.14 kDa which is too low. I've read so much I've gone cross eyed ( the reason I want to know is because kDa under 50 can increase inflammation rather than help it )...I'm hoping I'm on the right track!
 
Where are you getting these numbers from? Is it possible to link to the document or upload a picture?
 

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