PH Adjustment for Bacteria Media Using NaOH and H2SO4

  • Thread starter Thread starter princessdiana97
  • Start date Start date
  • Tags Tags
    Bacteria Ph
Click For Summary
SUMMARY

The discussion focuses on adjusting the pH of bacterial media using sodium hydroxide (NaOH) and sulfuric acid (H2SO4). A standard practice in the lab involves using a 5 M NaOH solution for effective pH adjustment, especially when HEPES buffer is present. The conversation highlights the importance of concentration; using a lower concentration may require larger volumes, while a higher concentration can lead to precision issues. The alternative acid commonly used is hydrochloric acid (HCl) at a concentration of 5 M, with other acids or bases typically at 1 M.

PREREQUISITES
  • Understanding of pH adjustment techniques
  • Familiarity with sodium hydroxide (NaOH) and hydrochloric acid (HCl)
  • Knowledge of buffer solutions, specifically HEPES
  • Basic laboratory safety protocols for handling strong acids and bases
NEXT STEPS
  • Research the properties and applications of HEPES buffer in microbiological media
  • Learn about the effects of different acid and base concentrations on pH adjustment
  • Explore alternative buffering agents for bacterial media
  • Investigate the use of pH meters for precise measurement in laboratory settings
USEFUL FOR

This discussion is beneficial for microbiologists, laboratory technicians, and researchers involved in preparing bacterial cultures and optimizing media pH levels.

princessdiana97
Messages
1
Reaction score
0
Hi I just need to know if there was a standard concentration for the acid and base that I am going to use to change the pH of the media that I am making for bacteria? I am using NaOH and H2SO4. THanks
 
Chemistry news on Phys.org
there not standard but you have to be carefull with the contrentration you will use. for instance in our lab to adjust the pH we use a 5 M NaOH solution. We also have HEPES buffer in our medium. This works works well because you do not have to had a large volume to change the pH. If you do not have buffers in your medium, a lower concentration migth be more suited. If your acid or base use for adjusting your pH is too low you will increase your volume significantly and might pass the targeted volume. If it is too strong you have problem adjusting precisely but you can always dilute high concentration of a strong acid/base in a becker to have a lower concentration.

In our we don't use H2SO4, we usually use HCl at around a 5 M concentration. Other acid or base we usually use a 1 M concentration.
 

Similar threads

Henderson Hasselbalch Equation - Request for Clarification
  • · Replies 8 ·
Replies
8
Views
2K
How do I calculate the pH of slime and its effect on viscosity?
  • · Replies 2 ·
Replies
2
Views
3K
Reaction between hydrogen halide and sulfuric acid
  • · Replies 6 ·
Replies
6
Views
3K
Chemistry How much NaOH I have to add to increase pH?
  • · Replies 3 ·
Replies
3
Views
6K
Synthesis of Fluorescents AuNCs
  • · Replies 1 ·
Replies
1
Views
2K
Controlling pH of solution without changing its conductivity
  • · Replies 3 ·
Replies
3
Views
4K
Something that doesn't make sense to me about calculating the pH
  • · Replies 14 ·
Replies
14
Views
3K
Groundwater Chemistry on an Island
  • · Replies 2 ·
Replies
2
Views
2K
What is the Boehm Back Titration end point?
  • · Replies 5 ·
Replies
5
Views
2K
Chemistry Moles of NaOH to Create Buffer Solution pH=6 in 0.42M Ethanoic Acid
  • · Replies 3 ·
Replies
3
Views
3K