Plasmid DNA from Pfizer COVID Vaccine Manufacturing

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Discussion Overview

The discussion centers around the findings presented by Dr. Phillip Buckhaults regarding DNA fragments found in batches of the Pfizer COVID-19 vaccine, specifically focusing on the implications of plasmid DNA used in vaccine manufacturing. The conversation touches on regulatory concerns, public health implications, and the potential for misinformation in the context of vaccine hesitancy.

Discussion Character

  • Debate/contested
  • Technical explanation
  • Meta-discussion

Main Points Raised

  • Dr. Buckhaults reported finding plasmid DNA in Pfizer vaccine batches, which he claims were not present in early studies.
  • He noted that the DNA levels are near regulatory limits and that the encapsulation in lipids complicates regulatory assessments.
  • Dr. Buckhaults suggested that there should be further investigation into whether plasmid DNA has integrated into the genomic DNA of vaccinated individuals.
  • Some participants express concern that this information could be misused by anti-vaccine advocates.
  • There is mention of a potential need for regulatory changes regarding the presence of DNA in mRNA vaccines.

Areas of Agreement / Disagreement

Participants do not reach a consensus on the implications of Dr. Buckhaults' findings, with some expressing concern about the potential for misinformation while others focus on the scientific and regulatory aspects of the findings.

Contextual Notes

Dr. Buckhaults' study is described as ad hoc and not peer-reviewed, which may affect the interpretation of his findings. The discussion reflects varying levels of concern regarding the regulatory framework governing mRNA vaccines and the implications of the findings for public health.

.Scott
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TL;DR
Dr. Buckhault using his own funds and with assistance from USC students and facilities, took advantage of the opportunity to check mRNA vaccine batches for DNA fragments. He found them and his concerns, taken at face value, seem valid and reasonable to me.
Last week, Phillip Buckhaults, PhD addressed the South Carolina Senate on the subject of DNA fragments the USC lab has found in batches of the Pfizer COVID-19 vaccines. Those fragments are from plasmids used by Pfizer in the large-scale manufacturing of their vaccine - but not in the early batches used in their early studies.

His address was about 34 minutes in length and is included in a "News Break" news article.

His study was ad hoc and he does not believe his finding rise to the quality level of peer-review publication.

Dr. Buckhaults is a professor at the University of South Carolina. His lab research has focused on "Determining biochemical mechanisms by which germline variants contribute to cancer health disparities that exist between people of different ethnic ancestries" and "Using genome sequencing and functional genomics to develop novel personalized medicine approaches to treat colon cancer".

He said that the DNA levels in the Pfizer vaccine batches are near the regulatory limit - in some batches a bit over. But the DNA is encapsulated in lipids and those limits are not appropriately addressed in the FD regulations. He also found DNA in the Moderna vaccine, but at lower levels.

His conclusions (from his PowerPoint):
We should check a bunch of vaccinated people to see if plasmid DNA has integrated into their genomic DNA.
We (you [government]) should insist that the USFDA force Pfizer to get the DNA out of the booster and all future mRNA based vaccines.
The regulation that allowed the DNA to be there should be changed. It's a leftover from previous vaccines that contained only naked DNA. The mRNA vaccines have this DNA encapsulated in a lipid nanoparticle delivery system (trojan horse) and so the DNA is a far more serious issue.
...

I very much expect that this will become an unavoidable public conversation.
Although Dr. Buckhaults is supportive of COVID vaccination, and expressed his support in his address, I have already seen postings that are using his address to argue against the programs - in terms we are probably all well-familiar with.
 
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We were having a brief conversation about this earlier this week. It's definitely fuel for any anti vaxxers to get carried away with until more information is determined.

Meanwhile, while it gave excuse for a few to complain about why their own (intentional) transfections weren't working lol.
 

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