Preparation of standard stock solution

In summary, the experiment involves creating a standard stock solution from lyphochek serum with a concentration of 6.8 g/dL. By pipetting 1.36 mL of the serum into a 25 mL volumetric flask and diluting it with physiological water, the resulting standard stock solution will have a concentration of 0.0036992 g/mL or 369.92 mg/dL. The calculation is correct and significant figures may vary depending on preference. The use of dL units is not necessary.
  • #1
Nanu Nana
95
5

Homework Statement


In this experiment we will create a standard stock solution from lyphochek serum. The concentration of proteins in serum Lyphochek is 6,8 g / dl. Pipette 1.36 mL Lyphochek serum in a 25 mL volumetric flask and dilute with physiological water. Calculate the standard stock solution concentration that you will make.

Homework Equations


c=n/v[/B]

The Attempt at a Solution


6,8 g/dl = 0,068 g/mL
0,068 g/mL x 1,36 mL = 0,09248 g / 25 mL = 0,0036992 g/mL
369,92 mg/ dl is the answer

is this answer correct ?? [/B]
 
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  • #2
Logic is OK, but where did you got so many significant figures from?
 
  • #3
Significant figures doesn't really matter here I think
 
  • #4
Borek said:
Logic is OK, but where did you got so many significant figures from?
thank you for your answer
 
  • #5
370 is all you'll ever need.
Are you forced to use horrible, unnecessary dL units?
 
  • #6
epenguin said:
370 is all you'll ever need.
Are you forced to use horrible, unnecessary dL units?
Not really . I just wanted to know if my calculation was correct or not
 

Related to Preparation of standard stock solution

What is a standard stock solution?

A standard stock solution is a solution with a known concentration that is used as a reference for preparing other solutions. It is typically made by dissolving a known mass of a pure substance in a known volume of solvent.

Why is it important to prepare a standard stock solution?

Preparing a standard stock solution is important because it ensures accuracy and consistency in experiments. By using a known concentration as a reference, scientists can accurately determine the concentration of unknown solutions and obtain reliable results.

How do you prepare a standard stock solution?

To prepare a standard stock solution, first determine the desired concentration and volume. Then, weigh out the appropriate amount of the pure substance and dissolve it in a specific volume of solvent. Finally, dilute the solution to the desired volume, making sure to mix thoroughly.

What is the difference between a primary and secondary standard stock solution?

A primary standard stock solution is a highly pure compound that can be used to make secondary standard solutions. Secondary standard solutions are made from primary standard solutions and are used for routine laboratory work. Primary standards have a higher level of accuracy and purity compared to secondary standards.

How do you determine the concentration of a standard stock solution?

The concentration of a standard stock solution can be determined by using a technique called titration, where a known volume of the solution is reacted with a known volume of a titrant until the reaction is complete. The amount of titrant used can then be used to calculate the concentration of the stock solution.

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