SUMMARY
To prepare a 250 mL acetic acid/acetate buffer at pH 5.6, the Henderson-Hasselbalch equation is essential for calculating the required concentrations of acetic acid and sodium acetate. Using the equation, the ratio of acetate to acetic acid can be determined, allowing for precise measurements. Specifically, 25.0 mL of glacial acetic acid (density 1.05 g/mL) is needed to achieve the desired buffer concentration. This method ensures that the buffer maintains its pH effectively within the specified range.
PREREQUISITES
- Understanding of the Henderson-Hasselbalch equation
- Knowledge of buffer solutions and their preparation
- Familiarity with molarity and concentration calculations
- Basic skills in laboratory measurement techniques
NEXT STEPS
- Study the Henderson-Hasselbalch equation in detail
- Learn about buffer capacity and its implications in biochemical applications
- Explore the preparation of different buffer systems using various acids and salts
- Investigate the effects of temperature on buffer pH stability
USEFUL FOR
Chemistry students, laboratory technicians, and researchers involved in biochemical experiments requiring precise pH control will benefit from this discussion.