- #1
sameeralord
- 662
- 3
Hello everyone,
I'm not very good at this area, so I tried to do some questions to get better, but I have many confusions as a result. This is not homework, I hope you can help me. Thank you
These are true, false questions.
*Restriction fragment length polymorphism can be used to carry out prenatal diagnosis?
I understand the concept, so I think this is true, if you use fetal tissues.
*Gel electrophoresis can be used to measure the length of the DNA accurately?
I can see how if you have standard fragment of known size you can do this but how can the distance of a fragment exactly give the size of the molecule. In electrophoresis particles with different sizes can end up at the same place due to other factors like charge and resistance. So is this false.
*Genetic engineering does not theoretically provide a care for enzyme deficiency?
This is false right
*DNA polymerase is denatured when enzymatic extension takes place at 72 degrees celcius?
I know Taq DNA polymerase is used so it wouldn't denature like dna polymerase. But I'm not sure if normal DNA polymerase denatures at 72 or 90 when melting of dna takes place?
*Isolated DNA can be rapidly sequenced by either chemical method or enzymatic method?
Not sure
*Blotting and hybridization techniques are important in the identification of the specific DNA and RNA fragment?
Here is probing considered a form of hybridization. I think this is faily true, but I don't think blotting can use RNA. Can it
*Polyacrilamide gel are used to separate DNA fragements with more than 500 nucleotides?
polyacrylamide gels to separate DNA fragments differing by a single base-pair in length , that is what wiki says. But I still don't get the answer.
Thanks
I'm not very good at this area, so I tried to do some questions to get better, but I have many confusions as a result. This is not homework, I hope you can help me. Thank you
These are true, false questions.
*Restriction fragment length polymorphism can be used to carry out prenatal diagnosis?
I understand the concept, so I think this is true, if you use fetal tissues.
*Gel electrophoresis can be used to measure the length of the DNA accurately?
I can see how if you have standard fragment of known size you can do this but how can the distance of a fragment exactly give the size of the molecule. In electrophoresis particles with different sizes can end up at the same place due to other factors like charge and resistance. So is this false.
*Genetic engineering does not theoretically provide a care for enzyme deficiency?
This is false right
*DNA polymerase is denatured when enzymatic extension takes place at 72 degrees celcius?
I know Taq DNA polymerase is used so it wouldn't denature like dna polymerase. But I'm not sure if normal DNA polymerase denatures at 72 or 90 when melting of dna takes place?
*Isolated DNA can be rapidly sequenced by either chemical method or enzymatic method?
Not sure
*Blotting and hybridization techniques are important in the identification of the specific DNA and RNA fragment?
Here is probing considered a form of hybridization. I think this is faily true, but I don't think blotting can use RNA. Can it
*Polyacrilamide gel are used to separate DNA fragements with more than 500 nucleotides?
polyacrylamide gels to separate DNA fragments differing by a single base-pair in length , that is what wiki says. But I still don't get the answer.
Thanks
Last edited: