The separability of Hilbert spaces is crucial in Quantum Mechanics as it allows for the application of the Stone-von Neumann theorem, which ensures that any irreducible unitary representation of canonical commutation relations can be realized in a separable space. While actual observations occur in finite-dimensional spaces, Rigged Hilbert spaces, which are separable, are utilized to manage potentially infinite dimensions. These spaces converge under weak topology, facilitating the identification of necessary subsets for practical applications. The discussion highlights the mathematical foundation and implications of using separable spaces in both non-relativistic and relativistic quantum theories. Understanding these concepts is essential for advancing quantum mechanics and its applications.