How does heat affect TLC visualization with sulfuric acid?

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SUMMARY

Heating affects Thin Layer Chromatography (TLC) visualization by potentially causing the evaporation of the solvent or eluent, which can lead to the disappearance of sample spots. Elevated temperatures, particularly around 120°C, facilitate the charring of organic compounds when treated with sulfuric acid (H2SO4), enhancing visualization. The increased molecular motion at higher temperatures may also result in messier sample spots. Understanding these effects is crucial for accurate TLC analysis.

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Homework Statement



How does heating affect the spots on the TLC?

Homework Equations



None

The Attempt at a Solution



I have no clue, unless heating evaporates the solution and therefore the spots disappear?
or Perhaps the elutent evaporated due to heat rather then the solution?
 
Last edited:
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Heating may or maynot affect the components of the organic solvents which have been adsorbed in the adsorbent.

however, if the temp is high enough, then the solvent may vaporize.
 
Last edited:
oahbpn said:

Homework Statement



How does heating affect the spots on the TLC?

Homework Equations



None

The Attempt at a Solution



I have no clue, unless heating evaporates the solution and therefore the spots disappear?
or Perhaps the elutent evaporated due to heat rather then the solution?

The higher temperature may (just a guess) make the sample spot become messier because of the increased random motion of the molecules of solvent. If this is correct, then maybe someone can transcribe into more precise language.
 
oahbpn said:

Homework Statement



How does heating affect the spots on the TLC?

Homework Equations



None

The Attempt at a Solution



I have no clue, unless heating evaporates the solution and therefore the spots disappear?
or Perhaps the elutent evaporated due to heat rather then the solution?

Is this question being asked in context with another question? Heating a TLC is performed sometimes after treatment with H2SO4. Did you treat the TLC with H2SO4?
 
yes you are correct , chemisttree

sorry about that
 
It's called visulization by charring. The sulfuric acid will catalyze the charring of organic compounds at elevated temperatures (~120C).
 
Last edited:

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