Chromatography Definition and 6 Discussions

Chromatography is a laboratory technique for the separation of a mixture.
The mixture is dissolved in a fluid (gas, solvent, water, ...) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which is fixed a material called the stationary phase. The different constituents of the mixture have different affinities for the stationary phase. The different molecules stay longer or shorter on the stationary phase, depending on their interactions with its surface sites. So, they travel at different apparent velocities in the mobile fluid, causing them to separate. The separation is based on the differential partitioning between the mobile and the stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation.Chromatography may be preparative or analytical. The purpose of preparative chromatography is to separate the components of a mixture for later use, and is thus a form of purification. Analytical chromatography is done normally with smaller amounts of material and is for establishing the presence or measuring the relative proportions of analytes in a mixture. The two are not mutually exclusive.

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  1. S

    Question about antibody affinity chromatography

    I am learning about protein purification in my Biochemistry class, and I have a question about protein elution in antibody affinity chromatography. My understanding of the mechanism for the technique is that proteins that do not bind to the antibody will be separated first as it runs down the...
  2. M

    MATLAB Chromatography PDE MOL

    Hello all I am using the method of lines to solve the following PDE: ## \frac {\partial C} {\partial t} + F\frac {\partial q} {\partial t} + u \frac {dC} {dz} = D_{ax} \frac{\partial^2 C} {\partial z^2} ## ## \frac {\partial q} {\partial t} = k (q^{*}-q) ## With these initial conditions: ##...
  3. E

    Latent Heat and Bond Energy

    The kinetic theory says that temperature is the measure of the average kinetic energy. That would mean then : The temperature rises with the rise in kinetic energy. We know that when matter changes state 'latent heat' helps in 'breaking the bonds' at the transition phase and that this 'extra...
  4. E

    Chromatography: The difference in kinetic energy

    In chromatography, the upper portion of the moving water solvent has more kinetic energy, than the lower portion of the moving water solvent because the lower portion of water molecules have to face more obstacles in the way because of the absorbed water molecules. Is it true?
  5. MarcozXD

    Should carbon boiling stones be used in O-chem reactions?

    I performed column cromatography on an oil, and used carbon boiling chips but got some weird IR. I used hexanes and acetone as solvents, I am trying to figure out some of the spikes, soo I am thinking that maybe the boiling stone being carbon could acted as activated carbon and absorbed some...
  6. D

    Separation Through chromatography

    Dear all, We all know that chromatography is a separation technique. In paper or TLC,the mixture is differentiated according to their relative affinities with the solvent on the paper or glass plate. How do we really separate the mixture. Kindly, help me how to extract the components...