Understanding Enzyme Kinetics: Calculating Vmax and Km

[marksju]

So, we were given a set of data of (uM) and the Velocity (umol/minute) of an inhibitor and no inhibitor.

we are supposed to find the values of Vmax and Km in the absence of inhibitor and in the presence.

do I use 1/Vo = 1/Vmax + Km/Vmax - 1/?

but what is Km and the intial velocity?

I'm sure this is fundamental for many, but the help would be appreciated.

 

[marksju]

\frac{1}{V{o}}=\frac{1}{V{max}}+\frac{K{m}}{V{max}}-\frac{1}{<s>}</s>

 

[marksju]

Using that equation, wouldn't we need to know what either K{m} or V{max} is?