Understanding Spectral Bandpass of Monochromator

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SUMMARY

The spectral bandpass of a monochromator refers to the range of wavelengths that can pass through the device after dispersion. Commercial fluorometers allow for a bandpass adjustment between 0.5-30 nm, where increasing the bandpass enhances light throughput by a factor of four but decreases resolution. Analysts can modify the bandpass to achieve an optimal balance between sensitivity and resolution during analysis.

PREREQUISITES
  • Understanding of monochromators and their function in spectroscopy
  • Familiarity with the concept of spectral resolution
  • Knowledge of light throughput and its impact on analytical results
  • Basic principles of fluorescence and fluorometry
NEXT STEPS
  • Research the impact of bandpass settings on spectral resolution in monochromators
  • Explore the design and function of commercial fluorometers
  • Learn about the relationship between light throughput and analytical sensitivity
  • Investigate methods for optimizing bandpass in fluorescence spectroscopy
USEFUL FOR

Researchers, analytical chemists, and laboratory technicians involved in spectroscopy and fluorescence analysis will benefit from this discussion.

tigger
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Hi.. can anyone please explain to me what does it mean by spectral bandpass of monochromator? i tried to look for it in my textbook, but i can't really find anything..
what i think is that this spectral bandpass gives you the size (wavelength range) of the wave component (after it passed the dispersive device) that can go through the monochromator... so when you do the analysis u get a band with this wavelength range.. is that correct? :confused:

Thanks in advance!
 
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Hello,

I have found the following from http://www.pti-nj.com/tech_3.html :

"Spectral bandpass of the monochromators. The bandpass of commercial fluorometers may be varied between 0.5-30 nm. Doubling the bandpass of a monochromator will increase the throughput of light by a factor of four. Resolution, however, is worse at high bandpass. The bandpass may be adjusted by the analyst to balance sensitivity and resolution."
 
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thank you so much! :smile:
 

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