No. Conservation of energy (i.e.) requires that the total amount of energy in the universe stays constant. If you take quantum mechanics into account, it might be better to phrase it as, 'the measured amount of energy in the universe must stay constant' (note that measurement doesn't just mean 'looking through a microscope and writing down a result, etc). Heisenberg's uncertainty principle states that the uncertainty in an energy measurement is related to the uncertainty in a time measurement:
[tex]
\Delta E \Delta t \geq \bar{h}/2[/tex]
Thus, virtual particles do not violate conservation of energy because the uncertainty in the energy of the system will always be large enough over the short time-periods under consideration.