A null set, also known as the empty set, is defined as a set that contains no elements, thus having a cardinality of zero. It serves to distinguish between "something" and "nothing" in mathematical contexts. The discussion highlights that the empty set is unique in its measure zero property, meaning it has no size or extent. Additionally, countable subsets of real numbers, such as the rationals, can also be considered null sets due to their measure zero. Understanding these concepts is essential for grasping more complex mathematical ideas related to sets and measures.