What is the best sterilization method for this growing medium?

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SUMMARY

The discussion centers on sterilization methods for a 1L solution of Murashige and Skoog micro- and macro-nutrients. The user inquires about the suitability of autoclaving, UVC sterilization, and filter sterilization for the solution, particularly regarding the stability of IAA and other compounds. It is concluded that autoclaving is a viable option, but caution is advised regarding nutrient degradation, especially with UVC sterilization. The user is also encouraged to consult manufacturers for sterilization guidance on macro-nutrients.

PREREQUISITES
  • Understanding of Murashige and Skoog nutrient composition
  • Knowledge of sterilization techniques including autoclaving and UVC sterilization
  • Familiarity with plant hormones such as IAA and their stability
  • Basic laboratory skills for preparing and handling nutrient solutions
NEXT STEPS
  • Research the autoclaving process for nutrient solutions, focusing on time and temperature settings
  • Investigate the effects of UVC sterilization on nutrient stability and efficacy
  • Learn about the filtration process using 0.22 µm pore filters for sterilizing solutions
  • Contact suppliers of macro-nutrients for best practices in sterilization and handling
USEFUL FOR

Researchers, plant biotechnologists, and laboratory technicians involved in plant tissue culture and nutrient preparation will benefit from this discussion.

Termotanque
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I have a 1l solution of Murashige and Skoog micro- and macro-nutrients. It also contains the vitamins and some hormones. The complete list is copied below. I emphasized the compounds that I think are the most unstable ones. Are the compounds listed autoclavable? I have anecdotal reports of only IAA not being autoclavable. Can you help me?

I want to sterilize this solution. Should I go with an autoclave? If so, for how long? Maybe UVC sterilization? Filter sterilization is not an option since I don't have access to a well-equipped lab.

Micro-nutrients:
* Ammonium nitrate (NH4NO3) 1,650 mg/l
* Boric acid (H3BO3) 6.2 mg/l
* Calcium chloride (CaCl2 · 2H2O) 440 mg/l
* Cobalt chloride (CoCl2 · 6H2O) 0.025 mg/l
* Magnesium sulfate (MgSO4 · 7H2O) 370 mg/l
* Cupric sulfate (CuSO4 · 5H2O) 0.025 mg/l
* Potassium phosphate (KH2PO4) 170 mg/l
* Ferrous sulfate (FeSO4 · 7H2O) 27.8 mg/l
* Potassium nitrate (KNO3) 1,900 mg/l
* Manganese sulfate (MnSO4 · 4H2O) 22.3 mg/l
* Potassium iodide (KI) 0.83 mg/l
* Sodium molybdate (Na2MoO4 · 2H2O) 0.25 mg/l
* Zinc sulfate (ZnSO4·7H2O) 8.6 mg/l
* Na2EDTA · 2H2O 37.2 mg/l

Macro-nutrients:
* i-Inositol 100 mg/l
* Niacin 0.5 mg/l
* Pyridoxine · HCl 0.5 mg/l
* Thiamine · HCl 0.1 mg/l
* IAA 1–30 mg/l
* Kinetin 0.04–10 mg/l
* NAA
* BAP
* Glycine (recrystallized) 2.0 g/l
* Sucrose 20 g/l
* Agar 10 g/l
 
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I would expect UV sterilization to degrade the nutrients. I would just filter the solution through a 0.22 um pore filter- it's really not that difficult to set up. AFAIK, none of your nutrients will stick to the filter.
 
also, the mfgr's of your macro nutrients most certainly can tell you how, or even supply you with previously-sterilized quantities