So we are learning about vectors and how you can insert vectors into a plasmid and then that plasmid in a bacteria will make lots of copies of the protein that your sequence codes for. I don't understand how inserting the DNA that codes for a protein into a bacteria gives you just the protein you want. Now, instead of just your DNA that codes the protein you want, that segment is buried within another sequence of bacterial DNA. So wouldn't a new protein be made? How is the same protein made from a sequence that is now just the middle of a larger sequence of different DNA? Do you have to bind the vector to a stop codon on both sides or something so only that fragment codes for the amino acids that make the protein? Also, one more question that may be relevant. Does a strand of DNA code for many different proteins on one strand? It must, or our 23 chromosomes would only make 23 proteins. So maybe the answer to my question is that the protein can be made from this small segment on the plasmid while the other parts of the sequence make different proteins, and the portions of the sequence do not form one giant protein.