Which Antibiotic for Selecting Mammalian Cells: Ampicillin or Neomycin?

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SUMMARY

The discussion focuses on the selection of mammalian cells using antibiotics, specifically comparing ampicillin and neomycin. It is established that neomycin is the appropriate choice for selection since mammalian cells lack a cell wall, which is the target of ampicillin. The conversation highlights that most eukaryotic cells exhibit resistance to the concentrations of antibiotics typically effective against bacteria, necessitating higher concentrations for effective selection.

PREREQUISITES
  • Understanding of antibiotic mechanisms, specifically ampicillin and neomycin
  • Knowledge of mammalian cell biology and structure
  • Familiarity with eukaryotic cell resistance to antibiotics
  • Basic concepts of vector design in molecular biology
NEXT STEPS
  • Research the mechanisms of action of neomycin in eukaryotic cells
  • Study the design and use of selection markers in mammalian cell vectors
  • Explore antibiotic resistance mechanisms in eukaryotic cells
  • Learn about optimal antibiotic concentrations for mammalian cell selection
USEFUL FOR

This discussion is beneficial for molecular biologists, genetic engineers, and researchers working with mammalian cell cultures who need to understand antibiotic selection strategies.

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if my vector has an amphicilin and a neomycin selection markers. which one should i use for selection of my mammalian cells?

thanks.
 
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You should understant the site of attack of the antibiotic you mention. Ampicilin attacks the cell wall synthesis whereas neomycin attacks the ribosome.

Mammalian cell do not have cell wall. The answer is neomycin.

Also most eukarytics cell are resitance to concentration of antibiotics used for bacteria. High concentration are required for selection.
 

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