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biologyquestions
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why dna absorbs best at 260nm?
Why Does DNA Absorb Best at 260nm in Molecular Biology?
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DNA absorbs best at 260nm due to the presence of aromatic bases, particularly adenine and guanine, which have strong absorbance at this wavelength. This absorbance is crucial for quantifying DNA concentration in molecular biology applications. The peak absorbance at 260nm allows researchers to assess the purity and quality of DNA samples effectively. Additionally, the absorbance at this wavelength is significantly higher than at other wavelengths, making it a standard measurement for nucleic acid quantification. Understanding this property is essential for accurate DNA analysis in various biological studies.
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berkeman
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Welcome to PF. 
What have you found so far in your searching? What do you find when you use that phrase in a Google search?biologyquestions said: