Why Does DNA Absorb Best at 260nm in Molecular Biology?

Thread starter biologyquestions
Start date Apr 3, 2023
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Biology Molecular Molecular biology Photometry

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DNA absorbs best at 260nm due to the presence of aromatic bases, particularly adenine and guanine, which have strong absorbance at this wavelength. This absorbance is crucial for quantifying DNA concentration in molecular biology applications. The peak absorbance at 260nm allows researchers to assess the purity and quality of DNA samples effectively. Additionally, the absorbance at this wavelength is significantly higher than at other wavelengths, making it a standard measurement for nucleic acid quantification. Understanding this property is essential for accurate DNA analysis in various biological studies.

Apr 3, 2023

biologyquestions

why dna absorbs best at 260nm?

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Apr 3, 2023

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why dna absorbs best at 260nm?

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