The discussion highlights that transfecting cells with miR-141 mimics results in increased expression of miR-141, which subsequently impairs insulin secretion at elevated glucose levels (16.7 mM). The conversation raises the question of whether the observed increase in miR-141 expression is due to RNA-dependent RNA polymerase (RDRP) activity, which is known to generate secondary miRNAs. However, it is noted that mammals, including mice and humans, lack RDRPs for amplifying small RNAs. Therefore, it is suggested that the RT-qPCR results may reflect the presence of both the miRNA mimics and the endogenous miRNA, rather than amplification through RDRP activity.