Main Question or Discussion Point
When performing transformation, after adding SOC media to the newly transformed cells, we place them at 37 degrees Celsius for an hour to allow growth. I understand the need for an incubator but I'm confused regarding how shaking helps in microbial growth? I did some searching on the internet and apparently shaking or rotating gives us a 2-fold higher transformation efficiency. Ok. But why? Can some one give a logical explanation for this. It makes sense to think that it would be easier for bacterial cells to divide if they were'nt being shaken. But the facts suggests otherwise. Any idea why?