Beer lambert equation and concentration

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SUMMARY

The discussion focuses on calculating the percentage of nitrogen by mass in a cocaine sample using the Beer-Lambert Law. A 15.23 mg sample was digested and diluted, with absorbance readings taken at 625 nm. A standard solution of NH4Cl was prepared for calibration, yielding absorbance values of 0.140 for the reagent blank, 0.308 for the NH4Cl standard, and 0.592 for the cocaine solution. The Beer-Lambert Law equation, A = εCL, is essential for determining the molar absorptivity and correlating the ammonium concentrations.

PREREQUISITES
  • Understanding of the Beer-Lambert Law (A = εCL)
  • Knowledge of absorbance measurement techniques at 625 nm
  • Familiarity with preparing standard solutions and dilutions
  • Basic chemistry concepts related to nitrogen and ammonia
NEXT STEPS
  • Calculate molar absorptivity (ε) using the standard NH4Cl solution data
  • Determine the concentration of ammonia in the cocaine solution using absorbance
  • Convert the ammonia concentration to nitrogen percentage by mass
  • Review the principles of spectrophotometry for accurate absorbance readings
USEFUL FOR

Chemists, laboratory technicians, and students involved in analytical chemistry, particularly those working with spectrophotometric analysis and nitrogen quantification in samples.

Scarpetta
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Please help, A 15.23mg sample of cocaine was cemically digested to convert its nitorgen to ammonia and then diluted to 100.00cm3. A 10.00cm3 of this solution was placed in a 50.00cm3 volumetric flask and treated with 5.0cm3 of phenol solution and 2.0cm3 of sodium hypochlorite solution. The sample was diluted to 50.00cm3 and the absorbance at 625nm was measured using a 1cm cell using water as a reference .

For calibration a standard solution was prepared by weighing 0.0100g of NH4CL into 1000.00cm3 flask and making up to volume with water. A 10.00cm3 aliquote of this solution was placed in a 50.00cm3 slask, treated with phenol and hypochlorite like the cocaine solution and made up to volume with water. The absorbance of this solution at 625nm was measured using a 1cm cell and water as a reference.

absorbance readings
Reagent blank 0.140
NH4CL standard 0.308
cocaine solution 0.592

I have to calculate the % of nitrogen by mass in the cocaine!

Can anyone explain in sensible terms how to do the calulation, i only know that the beer lambert law equation is used A=ECL / A=ECL

= A=C/A=C...:cry: :cry: :cry:
 
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As a general procedure, you'll need to obtain the molar absorptivity value from the standard. You can subsequently correlate the ammonium concentration values for the standard as well as the digested cocaine solution.
 

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